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17 March 2023 Fibre optic illuminated fluorescence microscopy with ultraviolet surface excitation
Alexander Yong, Zesheng Zheng, Chiyo Tan, Joel Ang, Jessica Kng, Ko Hui Tan, Cyrus Tan, Rachael Soh, Rachel Tan, Kaicheng Liang
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Proceedings Volume PC12363, Multiscale Imaging and Spectroscopy IV; PC123630T (2023) https://doi.org/10.1117/12.2648654
Event: SPIE BiOS, 2023, San Francisco, California, United States
Abstract
We built a fibre optic illuminated deep ultraviolet (DUV) fluorescence microscope. Our microscope was designed to be compact and portable, sitting on a 30cm by 30cm breadboard. Multimode fibres were magnetically butt coupled to 2 DUV light emitting diodes, which provided oblique epi-illumination of biological samples with short working distance objectives. The stage was motorised by stepper motors and controlled by a microcontroller which allowed for high-resolution imaging of large, fresh, and unsectioned tissue with high-throughput. We demonstrated the fluorescence microscope across a wide range of biological samples from single cells and spheroids to large tumours and organ samples.
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Alexander Yong, Zesheng Zheng, Chiyo Tan, Joel Ang, Jessica Kng, Ko Hui Tan, Cyrus Tan, Rachael Soh, Rachel Tan, and Kaicheng Liang "Fibre optic illuminated fluorescence microscopy with ultraviolet surface excitation", Proc. SPIE PC12363, Multiscale Imaging and Spectroscopy IV, PC123630T (17 March 2023); https://doi.org/10.1117/12.2648654
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KEYWORDS
Fiber optics
Luminescence
Microscopy
Ultraviolet radiation
Deep ultraviolet
Microscopes
Rhodamine B
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