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The goal of this study was to analyze temperature dependent changes in NIR reflectance of ex vivo freshly excised human skin. A broadband halogen lamp was used as a light source. Remitted light was collected using an optical fiber and analyzed by a grating spectrometer. Samples were heated to and maintained at temperatures up to 60°C using a temperature control unit. The temperature was also independently monitored using a thermal camera. Consistent changes found include blue shift in water absorption peaks. This method may prove to be useful for monitoring tissue temperature during clinical procedures in real time.
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Tyler W. Iorizzo, Annikki Williams, Victor A. Neel M.D., Anna N. Yaroslavsky, "Temperature dependent changes in ex-vivo skin reflectance," Proc. SPIE PC12376, Optical Tomography and Spectroscopy of Tissue XV, PC123760K (7 March 2023); https://doi.org/10.1117/12.2650548