Assessment of the mechanical behavior of adherent cells currently lacks a commercial solution. Mechanical behavior is projected to play a key role in development, vascular diseases, cancer progression, and tissue engineering. Microscopes customized using a single-board computing ecosystem have created an opportunity to assess cellular mechanics quantitatively. The success of such customized technology solutions can be ensured with unified and cross-platform toolkits that enable guided and programmable data acquisition, data analysis, and results visualization. To address this need, we developed an Integrative Toolkit to Analyze Cellular Signals (iTACS), which can be obtained from https://github.com/IntegrativeMechanobiologyLaboratory/iTACS.
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