Real-time precision opto-control of chemical processes in live cells (Conference Presentation)

Matthew G. Clark; Gil Gonzalez; Yiyang Luo; Jesus Aldana-Mendoza; Mark Carlsen; Greg Eakins; Mingji Dai; Chi Zhang

doi:10.1117/12.2650400

15 March 2023 Real-time precision opto-control of chemical processes in live cells (Conference Presentation)

Matthew G. Clark, Gil Gonzalez, Yiyang Luo, Jesus Aldana-Mendoza, Mark Carlsen, Greg Eakins, Mingji Dai, Chi Zhang

Proceedings Volume PC12383, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XXI; PC1238306 (2023) https://doi.org/10.1117/12.2650400
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Precise control of biochemical reactions in live cells is a long-sought goal for researchers. Currently, there is no method that has the chemical selectivity, spatial accuracy, and temporal response to image and manipulate dynamic cellular processes simultaneously in real-time. We develop a novel technology, real-time precision opto-control (RPOC), that uses the optical signal generated during laser scanning imaging to control the pixel locations at which the opto-control laser is turned “on.” This optical signal is gated using comparator circuitry to command the 1st order output of an acousto-optic modulator for laser activation to minimize off-target manipulation.

Conference Presentation

Citation Download Citation

Matthew G. Clark, Gil Gonzalez, Yiyang Luo, Jesus Aldana-Mendoza, Mark Carlsen, Greg Eakins, Mingji Dai, and Chi Zhang "Real-time precision opto-control of chemical processes in live cells (Conference Presentation)", Proc. SPIE PC12383, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XXI, PC1238306 (15 March 2023); https://doi.org/10.1117/12.2650400

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