Volumetric bond-selective imaging and spectroscopic analysis of cellular Tau fibrils through a fluorescence-guided computational chemical microscope (Conference Presentation)

Jian Zhao; Lulu Jiang; Yihong Xu; Alex C. Matlock; Jiabei Zhu; Lei Tian; Benjamin Wolozin; Ji-Xin Cheng

doi:10.1117/12.2647973

15 March 2023 Volumetric bond-selective imaging and spectroscopic analysis of cellular Tau fibrils through a fluorescence-guided computational chemical microscope (Conference Presentation)

Jian Zhao, Lulu Jiang, Yihong Xu, Alex C. Matlock, Jiabei Zhu, Lei Tian, Benjamin Wolozin, Ji-Xin Cheng

Author Affiliations +

Proceedings Volume PC12392, Advanced Chemical Microscopy for Life Science and Translational Medicine 2023; PC1239203 (2023) https://doi.org/10.1117/12.2647973
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

To uncover the pathological mechanism of neurodegenerative diseases, simple and cost-effective analytical methods are highly desirable to obtain biochemistry and morphology information on Tau protein aggregation. Here, we demonstrate the fluorescence-guided computational mid-infrared photothermal microscope, termed Fluorescence-guided Bond-selective Intensity Diffraction Tomography (FBS-IDT). FBS-IDT enables hyperspectral 3D bond-selective refractive index map recovery on Tau fibrils in cells. Depth-resolved mid-infrared fingerprint spectra extraction and related secondary protein structure analysis are demonstrated. 3D reconstruction of β sheet in Tau fibril is realized mainly based on the mid-infrared photothermal effects.

Conference Presentation

Citation Download Citation

Jian Zhao, Lulu Jiang, Yihong Xu, Alex C. Matlock, Jiabei Zhu, Lei Tian, Benjamin Wolozin, and Ji-Xin Cheng "Volumetric bond-selective imaging and spectroscopic analysis of cellular Tau fibrils through a fluorescence-guided computational chemical microscope (Conference Presentation)", Proc. SPIE PC12392, Advanced Chemical Microscopy for Life Science and Translational Medicine 2023, PC1239203 (15 March 2023); https://doi.org/10.1117/12.2647973

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