Chemical-specific precision control of biomolecules in live cells (Conference Presentation)

Matthew G. Clark; Gil A. Gonzalez; Yiyang Luo; Jesus Aldana-Mendoza; Mark Carlsen; Gregory Eakins; Mingjie Dai; Chi Zhang

doi:10.1117/12.2647403

15 March 2023 Chemical-specific precision control of biomolecules in live cells (Conference Presentation)

Matthew G. Clark, Gil A. Gonzalez, Yiyang Luo, Jesus Aldana-Mendoza, Mark Carlsen, Gregory Eakins, Mingjie Dai, Chi Zhang

Author Affiliations +

Proceedings Volume PC12392, Advanced Chemical Microscopy for Life Science and Translational Medicine 2023; PC123920G (2023) https://doi.org/10.1117/12.2647403
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

The capability to precisely control biomolecules in live cells would give a better understanding of site-specific biofunctions. We developed a real-time precision opto-control (RPOC) platform which uses chemical-specific optical signals to trigger another laser beam only at desired locations to control biomolecules with submicron precision, nanosecond response time, and high chemical specificity. Stimulated Raman scattering and fluorescence signals are used for chemical detection. Using RPOC, we demonstrate real-time and selective control of chemical states of photochromic molecules at different subcellular locations. When used with photoswitchable inhibitors, RPOC can control intercellular microtubule polymerization and lipid droplet dynamics site-specifically based on chemical preference.

Conference Presentation

Citation Download Citation

Matthew G. Clark, Gil A. Gonzalez, Yiyang Luo, Jesus Aldana-Mendoza, Mark Carlsen, Gregory Eakins, Mingjie Dai, and Chi Zhang "Chemical-specific precision control of biomolecules in live cells (Conference Presentation)", Proc. SPIE PC12392, Advanced Chemical Microscopy for Life Science and Translational Medicine 2023, PC123920G (15 March 2023); https://doi.org/10.1117/12.2647403

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