Mapping enzyme activity in cells and in vivo by mid-infrared photothermal imaging of nitrile chameleons (Conference Presentation)

Hongjian He; Jiaze Yin; Zian Wang; Meng Zhang; Bing Xu; Ji-Xin Cheng

doi:10.1117/12.2657138

15 March 2023 Mapping enzyme activity in cells and in vivo by mid-infrared photothermal imaging of nitrile chameleons (Conference Presentation)

Hongjian He, Jiaze Yin, Zian Wang, Meng Zhang, Bing Xu, Ji-Xin Cheng

Author Affiliations +

Proceedings Volume PC12392, Advanced Chemical Microscopy for Life Science and Translational Medicine 2023; PC123920S (2023) https://doi.org/10.1117/12.2657138
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Enzymes are vital in most physiological and biochemical processes and may function as critical biomarkers of disease. Notably, many biological events and signaling networks involve multiplex enzyme species. Thus, mapping multiple enzyme activities is significant for elucidating enzymatic functions in life and disease. Here, we report a novel technology to map the catalytic efficacy of phosphatase and caspase in live cancer cells and in C. elegans by mid-infrared photothermal (MIP) imaging of nitrile chameleons. This technology will certainly bring new insight into the roles of enzyme in biochemical activities and explore new knowledge of enzymatic activity in health and disease.

Conference Presentation

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Hongjian He, Jiaze Yin, Zian Wang, Meng Zhang, Bing Xu, and Ji-Xin Cheng "Mapping enzyme activity in cells and in vivo by mid-infrared photothermal imaging of nitrile chameleons (Conference Presentation)", Proc. SPIE PC12392, Advanced Chemical Microscopy for Life Science and Translational Medicine 2023, PC123920S (15 March 2023); https://doi.org/10.1117/12.2657138

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KEYWORDS

In vivo imaging

Mid-IR

Cancer

Absorbance

Image resolution

In vitro testing

Infrared imaging

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