Dual frequency stimulated Raman microscopy (Conference Presentation)

Barbara Sarri; Sandro Heuke; Chang Liu; Hervé Rigneault; Ingo Rimke; Edlef Büttner

doi:10.1117/12.2648083

15 March 2023 Dual frequency stimulated Raman microscopy (Conference Presentation)

Barbara Sarri, Sandro Heuke, Chang Liu, Hervé Rigneault, Ingo Rimke, Edlef Büttner

Proceedings Volume PC12392, Advanced Chemical Microscopy for Life Science and Translational Medicine 2023; PC123921B (2023) https://doi.org/10.1117/12.2648083
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

We present a ready to use label free coherent Raman microscope (CARS/SRS) to perform instantaneous virtual histology and chemical imaging for scientific applications. The system is based on a picosecond laser source that can address two vibrational wavenumbers simultaneously across the entire vibrational spectrum (400cm-1 – 4000cm-1). The laser source is coupled to a ready to use beam scanning microscope enabling SRS, CARS and multiphoton microscopy. Targeting the CH2 and CH3 chemical bonds, we demonstrate instantaneous stimulated Raman histology in the brain and the GI. This new, ready to use, SRS/CARS and multiphoton microscope enables SRS detection with exquisite sensitivity (shot noise limited) and ratio-metric measurement by targeting simultaneously two vibrational frequencies that can be tuned anywhere across the vibrational spectrum.

Conference Presentation

Citation Download Citation

Barbara Sarri, Sandro Heuke, Chang Liu, Hervé Rigneault, Ingo Rimke, and Edlef Büttner "Dual frequency stimulated Raman microscopy (Conference Presentation)", Proc. SPIE PC12392, Advanced Chemical Microscopy for Life Science and Translational Medicine 2023, PC123921B (15 March 2023); https://doi.org/10.1117/12.2648083

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