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Bioprinting allows for the formation of three-dimensional living tissues through the precise layer-by-layer printing of biomaterials such as living cells and cell-laden hydrogels. In this study, Laser-Induced Forward Transfer (LIFT) printing was used to rapidly and accurately deposit patterns of cancer cells in a non-contact manner using two different wavelengths, 532 and 355nm. Overall, we find that LIFT is able to safely print patterns of breast cancer cells with high viability and little to no heat or shear damage to the cells, as indicated by unperturbed growth and negligible gross DNA damage.
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Evina Elezoglou, Panagiotis Karakaidos, Christina Kryou, Antonis Hatziapostolou, Apostolos Klinakis, Ioanna Zergioti, "LIFT printing of cells and DNA damage study," Proc. SPIE PC12412, Laser 3D Manufacturing X, PC1241203 (17 March 2023); https://doi.org/10.1117/12.2648064