Multimodal imaging of ciliary dynamics using optical coherence tomography and phase-resolved spectrally encoded interferometric microscopy

Wenqi He; Do A. Kim; Zhikai Zhu; Lidek Chou; Katelyn Dilley; Ellen Hong; Edward Kuan; Brian Wong; Zhongping Chen

doi:10.1117/12.3001959

13 March 2024 Multimodal imaging of ciliary dynamics using optical coherence tomography and phase-resolved spectrally encoded interferometric microscopy

Wenqi He, Do A. Kim, Zhikai Zhu, Lidek Chou, Katelyn Dilley, Ellen Hong, Edward Kuan, Brian Wong, Zhongping Chen

Author Affiliations +

Proceedings Volume PC12818, Imaging, Therapeutics, and Advanced Technology in Head and Neck Surgery and Otolaryngology 2024; (2024) https://doi.org/10.1117/12.3001959
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Proper ciliary dynamics is vital for effective mucociliary transport, the primary defense mechanism for the upper respiratory tract. Abnormal cilia behavior could lead to chronic respiratory disease, making it essential to conduct more detailed studies. In this study we present a multimodality system, specifically using optical coherence tomography (OCT) and phase-resolved spectrally encoded interferometric microscopy (PR-SEIM). We have already shown that PR-SEIM is capable of measuring cilia beat frequency ex vivo. Although we were able to visually identify the ciliary motion in the nasal cavity of rabbits in vivo, due to its sensitivity to motion artifacts, it has been difficult to quantitatively analyze ciliary dynamics. To overcome this obstacle, we incorporated OCT along with a high-speed laser source to compensate for bulk motion. Ultimately, this system will provide a way to study ciliary dynamics in its natural environment, thus allowing more in-depth understanding of ciliary functions.

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Wenqi He, Do A. Kim, Zhikai Zhu, Lidek Chou, Katelyn Dilley, Ellen Hong, Edward Kuan, Brian Wong, and Zhongping Chen "Multimodal imaging of ciliary dynamics using optical coherence tomography and phase-resolved spectrally encoded interferometric microscopy", Proc. SPIE PC12818, Imaging, Therapeutics, and Advanced Technology in Head and Neck Surgery and Otolaryngology 2024, (13 March 2024); https://doi.org/10.1117/12.3001959

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KEYWORDS

Phase interferometry

Optical coherence tomography

Microscopy

Multimodal imaging

Laser frequency

Laser sources

Motion analysis

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