Potassium selenocyanate potentiates both antimicrobial blue light and antimicrobial photodynamic inactivation against Gram-negative bacteria

Min Wu; Carolina dos Anjos; Jiaqi Weng; Tianhong Dai

doi:10.1117/12.3023279

13 March 2024 Potassium selenocyanate potentiates both antimicrobial blue light and antimicrobial photodynamic inactivation against Gram-negative bacteria

Min Wu, Carolina dos Anjos, Jiaqi Weng, Tianhong Dai

Author Affiliations +

Proceedings Volume PC12822, Photonic Diagnosis, Monitoring, Prevention, and Treatment of Infections and Inflammatory Diseases 2024; PC128220Z (2024) https://doi.org/10.1117/12.3023279
Event: SPIE BiOS, 2024, San Francisco, California, United States

Conference Poster

Abstract

This study investigated the potentiation of KSeCN for both antimicrobial blue light (aBL,405nm) and antimicrobial photodynamic inactivation (aPDI) against different species of Gram-negative bacteria. For planktonic (108CFU/ml) E.coli, with KSeCN treatment (50mM concentration and 60J/cm2 aBL) demonstrated an increase of 4- to 5-log10 CFU reduction compared to aBL alone. E. coli, with KSeCN treatment (50mM,100uM Verteporfin and 20J/cm2 aBL) demonstrated an increase of 4- to 6-log10 CFU reduction compared to aPDI (100uM concentration of Verteporfin and 20J/cm2 aBL, 30min incubation time) alone. K. Pneumoniae and A. Baumannii show similar results. For biofilm (106 CFU/mL), an obvious CFU reduction of 5- to 7-log10 could be seen with KSeCN treatment (50mM and 100J/cm2 aBL) compared to aBL alone. CFU reduction of 6.5- to 8-log10 could be seen with KSeCN treatment (50mM,100uM Verteporfin and 40J/cm2 aBL) compared to aPDI alone (100uM concentration of Verteporfin and 40J/cm2). Confocal results (live/dead) made these findings solid which highlight the KSeCN function to potentiate aBL and aPDI against G-bacteria, offering a novel approach for antibiotic resistance infections.

Citation Download Citation

Min Wu, Carolina dos Anjos, Jiaqi Weng, and Tianhong Dai "Potassium selenocyanate potentiates both antimicrobial blue light and antimicrobial photodynamic inactivation against Gram-negative bacteria", Proc. SPIE PC12822, Photonic Diagnosis, Monitoring, Prevention, and Treatment of Infections and Inflammatory Diseases 2024, PC128220Z (13 March 2024); https://doi.org/10.1117/12.3023279

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