Exploring neurovascular interactions in the retina using multi-view light-sheet microscopy

Enbo Zhu; Yaran Zhang; Jing Wang; Alison Chu; Tzung K. Hsiai

doi:10.1117/12.3000674

13 March 2024 Exploring neurovascular interactions in the retina using multi-view light-sheet microscopy

Enbo Zhu, Yaran Zhang, Jing Wang, Alison Chu, Tzung K. Hsiai

Proceedings Volume PC12824, Ophthalmic Technologies XXXIV; PC128241D (2024) https://doi.org/10.1117/12.3000674
Event: SPIE BiOS, 2024, San Francisco, California, United States

Conference Poster

Abstract

Vascular retinopathy is a leading cause of preventable blindness in diabetes. While most studies focus on individual neural or vascular structures, there remains a knowledge gap in understanding the retinal neurovascular interactions due to injury and repair. We seek to demonstrate 3-D neurovascular interactions with a multi-view light-sheet imaging system. Intact 3-D retinas from 2-month-old mice were fixed and permeabilized followed by the optical clearance. Retinal vessels and neuron cells were stained with fluorescent labels. After refractive index matching, multi-view light-sheet imaging was performed at different angles followed by 3-D registration and reconstruction. This enabled 3-D co-registration of the vasculature in relation to the neuron cells for virtualization. Therefore, the 3-D neuro-microvascular network could be revealed with both deep tissue penetration and high spatial resolution for investigating hyperoxia and diabetes-associated vascular retinopathy.

Citation Download Citation

Enbo Zhu, Yaran Zhang, Jing Wang, Alison Chu, and Tzung K. Hsiai "Exploring neurovascular interactions in the retina using multi-view light-sheet microscopy", Proc. SPIE PC12824, Ophthalmic Technologies XXXIV, PC128241D (13 March 2024); https://doi.org/10.1117/12.3000674

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