A novel pipeline for quantitative optical properties and fluorescence analysis of fresh human glioma tissue

Silvère Ségaud; Matthew Elliot; Bappaditya Debnath; Graeme Stasiuk; Tom Vercauteren; Yijing Xie; Jonathan Shapey

doi:10.1117/12.3001923

13 March 2024 A novel pipeline for quantitative optical properties and fluorescence analysis of fresh human glioma tissue

Silvère Ségaud, Matthew Elliot, Bappaditya Debnath, Graeme Stasiuk, Tom Vercauteren, Yijing Xie, Jonathan Shapey

Author Affiliations +

Proceedings Volume PC12828, Neural Imaging and Sensing 2024; PC128280F (2024) https://doi.org/10.1117/12.3001923
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

We present a novel pipeline for robust optical analysis of fresh human brain tissue. We capture fluorescence signal and characterize optical properties with high sensitivity and high spectral resolution. These in turn allow for quantitative analysis of protoporphyrin IX (PpIX) accumulation in various types of tumor tissue. Our ex vivo protocol for tissue handling was designed to promote high-fidelity replication of in vivo conditions. The on-going consolidation of a fresh ex vivo quantitative dataset from a cohort of 20 patients plus a control cohort lays foundation for the development of imaging devices for intraoperative fluorescence guided resection.

Conference Presentation

Citation Download Citation

Silvère Ségaud, Matthew Elliot, Bappaditya Debnath, Graeme Stasiuk, Tom Vercauteren, Yijing Xie, and Jonathan Shapey "A novel pipeline for quantitative optical properties and fluorescence analysis of fresh human glioma tissue", Proc. SPIE PC12828, Neural Imaging and Sensing 2024, PC128280F (13 March 2024); https://doi.org/10.1117/12.3001923

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available