Three-dimensional velocimetry based on a single OCT volume containing double B-scans for assessment of embryo movement in the mouse oviduct in vivo

Tianqi Fang; Huan Han; Jingyu Sun; Shang Wang

doi:10.1117/12.3010141

13 March 2024 Three-dimensional velocimetry based on a single OCT volume containing double B-scans for assessment of embryo movement in the mouse oviduct in vivo

Tianqi Fang, Huan Han, Jingyu Sun, Shang Wang

Author Affiliations +

Proceedings Volume PC12841, Dynamics and Fluctuations in Biomedical Photonics XXI; PC1284101 (2024) https://doi.org/10.1117/12.3010141
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

The unique 3D imaging scale of OCT enables high-resolution tracking of large-size particle over a millimeter-level field of view. However, limited volumetric imaging speed hinders the assessment of fast movement, where a significant sacrifice in spatial sampling or transverse scanning distance is often needed. To address this technical hurdle in the application of studying the transport of preimplantation embryos in the mouse oviduct (fallopian tube) in vivo, we developed a new velocimetry method that relies on the particle streak formed in a single OCT volume containing double B-scans at each B-scan location, providing 3D structure information with particle velocity.

Conference Presentation

Citation Download Citation

Tianqi Fang, Huan Han, Jingyu Sun, and Shang Wang "Three-dimensional velocimetry based on a single OCT volume containing double B-scans for assessment of embryo movement in the mouse oviduct in vivo", Proc. SPIE PC12841, Dynamics and Fluctuations in Biomedical Photonics XXI, PC1284101 (13 March 2024); https://doi.org/10.1117/12.3010141

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