Label-free cell-tracking-enabled super-resolution functional photoacoustic microscopy

Zhuoying Wang; Fenghe Zhong; Youngseop Lee; Jiaxiao Han; Naidi Sun; Ziang Feng; Shuo Yang; Shengyun Ji; Allison Martinez; Hao F. Zhang; Cheng Sun; Song Hu

doi:10.1117/12.3002750

13 March 2024 Label-free cell-tracking-enabled super-resolution functional photoacoustic microscopy

Zhuoying Wang, Fenghe Zhong, Youngseop Lee, Jiaxiao Han, Naidi Sun, Ziang Feng, Shuo Yang, Shengyun Ji, Allison Martinez, Hao F. Zhang, Cheng Sun, Song Hu

Author Affiliations +

Proceedings Volume PC12842, Photons Plus Ultrasound: Imaging and Sensing 2024; PC128420E (2024) https://doi.org/10.1117/12.3002750
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Exploiting the optical absorption of hemoglobin, photoacoustic microscopy (PAM) has achieved label-free imaging of the microvasculature in vivo and enabled simultaneous quantification of blood oxygenation and flow. However, the axial resolution of PAM is limited to the mesoscopic level due to the finite bandwidth of detected ultrasound signals. To address this limitation, we have developed a super-resolution functional PAM technique based on spatiotemporal tracking of red blood cells, which enables label-free functional microvascular imaging in 3D at the single-cell level. We have demonstrated the utility of this technique by imaging the mouse brain’s responses to a single-vessel stroke in 3D.

Conference Presentation

Citation Download Citation

Zhuoying Wang, Fenghe Zhong, Youngseop Lee, Jiaxiao Han, Naidi Sun, Ziang Feng, Shuo Yang, Shengyun Ji, Allison Martinez, Hao F. Zhang, Cheng Sun, and Song Hu "Label-free cell-tracking-enabled super-resolution functional photoacoustic microscopy", Proc. SPIE PC12842, Photons Plus Ultrasound: Imaging and Sensing 2024, PC128420E (13 March 2024); https://doi.org/10.1117/12.3002750

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