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13 March 2024 Combined ultraviolet confocal reflectance, autofluorescence, and photoacoustic remote sensing microscopy for generating virtual sectioned histology in thick tissues
Nathaniel J. M. Haven, Brendon S. Restall, Matthew T. Martell, Brendyn D. Cikaluk, Roger J. Zemp
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Proceedings Volume PC12842, Photons Plus Ultrasound: Imaging and Sensing 2024; PC128421K (2024) https://doi.org/10.1117/12.3002864
Event: SPIE BiOS, 2024, San Francisco, California, United States
Abstract
Pathologist evaluation of brightfield microscopy images of H&E-stained tissues is time-consuming and labor-intensive, preventing intraoperative feedback to surgeons. Combined ultraviolet reflectance and photoacoustic remote sensing (PARS) microscopy was recently introduced, providing a label-free way to obtain cell nuclei and cytoplasm images. Additionally, utilizing deep learning and fast voice-coil scanning, maximally realistic virtual histology can be rapidly generated with this technique. Here, we introduce combined ultraviolet confocal reflectance, autofluorescence, and PARS microscopy, utilizing a single 266 nm excitation beam. This system now allows us to generate sectioned virtual histology in thick tissues well suited to the intraoperative setting.
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Nathaniel J. M. Haven, Brendon S. Restall, Matthew T. Martell, Brendyn D. Cikaluk, and Roger J. Zemp "Combined ultraviolet confocal reflectance, autofluorescence, and photoacoustic remote sensing microscopy for generating virtual sectioned histology in thick tissues", Proc. SPIE PC12842, Photons Plus Ultrasound: Imaging and Sensing 2024, PC128421K (13 March 2024); https://doi.org/10.1117/12.3002864
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KEYWORDS
Confocal microscopy
Tissues
Autofluorescence
Reflectivity
Ultraviolet radiation
Photoacoustic spectroscopy
Remote sensing
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