Comparing deep ultraviolet transmission microscopy and ultraviolet photoacoustic remote sensing microscopy for thin-tissue histology

Brendyn D. Cikaluk; Mohammad H. Masoumi; Nathaniel J. M. Haven; Matthew T. Martell; Brendon S. Restall; Roger J. Zemp

doi:10.1117/12.3002868

13 March 2024 Comparing deep ultraviolet transmission microscopy and ultraviolet photoacoustic remote sensing microscopy for thin-tissue histology

Brendyn D. Cikaluk, Mohammad H. Masoumi, Nathaniel J. M. Haven, Matthew T. Martell, Brendon S. Restall, Roger J. Zemp

Author Affiliations +

Proceedings Volume PC12842, Photons Plus Ultrasound: Imaging and Sensing 2024; PC128421N (2024) https://doi.org/10.1117/12.3002868
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Ultraviolet photacoustic remote sensing (UV-PARS) microscopy is a non-contact, label-free imaging modality which has demonstrated the ability to generate virtual histology images that show good concordance to gold-standard hematoxylin and eosin (H&E) stains of fixed tissue sections. However, UV-PARS microscopy requires time-consuming and complex coalignment of multiple beams for imaging. In this work, we demonstrate UV transmittance and scattering microscopy for virtual histology and compare with UV-PARS microscopy. Maximally realistic virtual histology images are generated for both UV-PARS and UV-transmittance microscopy techniques using a cycle-consistent generative adversarial network (CycleGAN) and compared to one another alongside the gold-standard brightfield microscopy of H&E stained fixed tissues.

Conference Presentation

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Brendyn D. Cikaluk, Mohammad H. Masoumi, Nathaniel J. M. Haven, Matthew T. Martell, Brendon S. Restall, and Roger J. Zemp "Comparing deep ultraviolet transmission microscopy and ultraviolet photoacoustic remote sensing microscopy for thin-tissue histology", Proc. SPIE PC12842, Photons Plus Ultrasound: Imaging and Sensing 2024, PC128421N (13 March 2024); https://doi.org/10.1117/12.3002868

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