Stable 266 nm and high-repetition rate light source using second harmonic generation for ultraviolet-photoacoustic microscopy

Do-yeon Kim; Sang-Won Lee

doi:10.1117/12.3003381

13 March 2024 Stable 266 nm and high-repetition rate light source using second harmonic generation for ultraviolet-photoacoustic microscopy

Do-yeon Kim, Sang-Won Lee

Author Affiliations +

Proceedings Volume PC12842, Photons Plus Ultrasound: Imaging and Sensing 2024; PC1284221 (2024) https://doi.org/10.1117/12.3003381
Event: SPIE BiOS, 2024, San Francisco, California, United States

Conference Poster

Abstract

Histopathology is the fundamental microscopic examination tool in many domains including cancer diagnosis and surgical oncology, and various fields. Recently, the UV-PAM is powerful imaging system for label-free histology-like imaging. In histopathology, the nuclei in cancer cells have typical morphological features, such as irregular shapes and large sizes. Alternatively, UV-PAM can obtain unstrained cell nuclei with specific, positive, and high-contrast images. UV-PAM is useful histological images of unstained cell nuclei with specific, positive, and high-image contrast. By using the 266 nm light, high contrast photoacoustic images can be obtained. In the recent UV-PAM, the hardware has been faced a fundamental limitation to laser source especially. There is no available commercial nanosecond (ns) laser that has a high repetition rate operation of hundreds of kHz at 266 nm. Here, we develop a stable high-repetition rate 266 nm source using the basic second harmonic generation (SHG) phenomenon. This stable 266 nm source is based on a simplified architecture using single-pass SHG with BBO crystal.

Citation Download Citation

Do-yeon Kim and Sang-Won Lee "Stable 266 nm and high-repetition rate light source using second harmonic generation for ultraviolet-photoacoustic microscopy", Proc. SPIE PC12842, Photons Plus Ultrasound: Imaging and Sensing 2024, PC1284221 (13 March 2024); https://doi.org/10.1117/12.3003381

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