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13 March 2024 Bacterial behavior and biofilm formation in the presence of optical traps
Brooke Walter-Lakes, Czarlyn Camba, Sattar Taheri-Araghi, Anna S. Bezryadina
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Proceedings Volume PC12846, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XXII; PC128460L (2024) https://doi.org/10.1117/12.3002112
Event: SPIE BiOS, 2024, San Francisco, California, United States
Abstract
In response to a hostile environment, some bacteria form a biofilm by secreting a glue-like matrix called extracellular polymeric substance (EPS) that functions as a physical barrier. In this work, we study the biofilm formation of Bacillus subtilis within minimal biofilm-promoting media (MSgg) and how optical trapping affects bacteria aggregation and biofilm formation. We demonstrate that a laser with a low absorbent wavelength can be used to manipulate biofilm and aggregate bacteria without causing significant photodamage. Whereas, even at low power, a laser with a high absorbent wavelength disrupts biofilm formation and causes significant photodamage to trapped bacteria.
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Brooke Walter-Lakes, Czarlyn Camba, Sattar Taheri-Araghi, and Anna S. Bezryadina "Bacterial behavior and biofilm formation in the presence of optical traps", Proc. SPIE PC12846, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XXII, PC128460L (13 March 2024); https://doi.org/10.1117/12.3002112
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KEYWORDS
Biofilms
Bacteria
Optical tweezers
Laser development
Microfluidics
Microscopes
Near infrared
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