Topographic imaging of three-dimensional dynamic samples at 67 Hz

Narcís Vilar Sole; Roger Artigas; Martí Duocastella; Guillem Carles

doi:10.1117/12.3001005

13 March 2024 Topographic imaging of three-dimensional dynamic samples at 67 Hz

Narcís Vilar Sole, Roger Artigas, Martí Duocastella, Guillem Carles

Proceedings Volume PC12848, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXXI; PC128480G (2024) https://doi.org/10.1117/12.3001005
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Optical techniques enable the non-contact surface measurement of three-dimensional objects at sub-micrometer resolution. However, they typically require the acquisition of multiple images at different axial positions, a time-consuming task that strongly limits imaging speed. Here, we propose Encoded Search Focal Scan (ESFS), a method able to reconstruct sample height maps from an order-of-magnitude reduced set of images. We demonstrate ESFS measurements over axial ranges exceeding 100 µm at sub-micrometric resolution while using only 8 input images. This leads to striking reconstruction rates of 67 topographies per second, opening the door to the real-time characterization of large and rapidly moving systems.

Conference Presentation

Citation Download Citation

Narcís Vilar Sole, Roger Artigas, Martí Duocastella, and Guillem Carles "Topographic imaging of three-dimensional dynamic samples at 67 Hz", Proc. SPIE PC12848, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXXI, PC128480G (13 March 2024); https://doi.org/10.1117/12.3001005

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