Holotomography as a tool for identifying impaired stemness in induced pluripotent stem cells

Geon Kim; Hoewon Park; Jeongwon Shin; Ki-Jun Yoon; YongKeun Park

doi:10.1117/12.3008974

13 March 2024 Holotomography as a tool for identifying impaired stemness in induced pluripotent stem cells

Geon Kim, Hoewon Park, Jeongwon Shin, Ki-Jun Yoon, YongKeun Park

Proceedings Volume PC12852, Quantitative Phase Imaging X; PC128520P (2024) https://doi.org/10.1117/12.3008974
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Induced pluripotent stem cells (iPSCs) hold the potential for personalized regenerative medicine. Yet accurately gauging the stemness of each colony remains a challenge since existing methods are inconsistent or harmful to iPSCs. Addressing this, we introduce holotomography (HT), a non-invasive microscopic technique. HT, through three-dimensional refractive index distributions, revealed iPSC structures at various scales as well as properties like volume, mass density, and lipid ratio. We identified altered properties in iPSCs exposed to differentiation agents, and then employed a machine-learning algorithm to detect reduced stemness from images. Through these results, HT emerges as a potential tool for iPSC quality maintenance.

Conference Presentation

Citation Download Citation

Geon Kim, Hoewon Park, Jeongwon Shin, Ki-Jun Yoon, and YongKeun Park "Holotomography as a tool for identifying impaired stemness in induced pluripotent stem cells", Proc. SPIE PC12852, Quantitative Phase Imaging X, PC128520P (13 March 2024); https://doi.org/10.1117/12.3008974

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