Deep quantitative phase imaging using confocal gradient light interference microscopy for studying spheroids and in vivo mouse brains

Xi Chen; Mikhail E. Kandel; Shitong Zhao; Rick Zirkel; Kai-Yu Huang; Mariya Sokolova; Chris B. Schaffer; Hyun Joon Kong; Chris Xu

doi:10.1117/12.3001167

13 March 2024 Deep quantitative phase imaging using confocal gradient light interference microscopy for studying spheroids and in vivo mouse brains

Xi Chen, Mikhail E. Kandel, Shitong Zhao, Rick Zirkel, Kai-Yu Huang, Mariya Sokolova, Chris B. Schaffer, Hyun Joon Kong, Chris Xu

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Proceedings Volume PC12852, Quantitative Phase Imaging X; PC1285216 (2024) https://doi.org/10.1117/12.3001167
Event: SPIE BiOS, 2024, San Francisco, California, United States

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Abstract

We present a deep label-free quantitative phase imaging method using confocal gradient light interference microscopy (CGLIM) for studying ex vivo three-dimensional cellular clusters and in vivo mouse brains. CGLIM is a custom-built confocal reflectance differential interference contrast (DIC) microscope with phase-shifting interferometry using long-wavelength (~1.7 μm) illumination. As a laser-scanning common-path interferometric technique based on linear scattering, CGLIM has superior stability and sensitivity in phase measurement and reduces photodamage, or heating to living systems. With the proposed method, we will demonstrate the working principle on standard samples, and the phase imaging of cancer spheroids and in vivo mouse brains.

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

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Xi Chen, Mikhail E. Kandel, Shitong Zhao, Rick Zirkel, Kai-Yu Huang, Mariya Sokolova, Chris B. Schaffer, Hyun Joon Kong, and Chris Xu "Deep quantitative phase imaging using confocal gradient light interference microscopy for studying spheroids and in vivo mouse brains", Proc. SPIE PC12852, Quantitative Phase Imaging X, PC1285216 (13 March 2024); https://doi.org/10.1117/12.3001167

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