In understanding the relationship between neuronal structure and functionality, we leverage calcium/voltage imaging to record the propagation of neuronal signals in vivo. To aptly capture these functional dynamics, we present 2-photon Line Excitation Array Detection (2p-LEAD) microscopy. Through shaping the 1035nm excitation beam into a line, 2p-LEAD can operate at 3000 FPS, using a linear array of PMT’s to collect the resulting fluorescence. With the replacement of the scanning mirror for an acousto-optic deflector, 2p-LEAD holds the potential of achieving kilohertz volumetric imaging over the visual cortex of the mice brain.
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