Fluorescence-free super-resolution imaging and nano-environment sensing by assessing temporal evolutions of optical processes

Delong Zhang

doi:10.1117/12.3009658

13 March 2024 Fluorescence-free super-resolution imaging and nano-environment sensing by assessing temporal evolutions of optical processes

Delong Zhang

Author Affiliations +

Proceedings Volume PC12853, High-Speed Biomedical Imaging and Spectroscopy IX; PC128530D (2024) https://doi.org/10.1117/12.3009658
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

This presentation will center on the investigation of two distinct relaxation mechanisms: photothermal relaxation and vibrational dephasing. We introduce a fluorescence-independent super-resolution technique, photothermal relaxation localization (PEARL) microscopy. PEARL microscopy leverages the evaluation of absorption-induced local refractive index modulation to extract intricate spatial details. Notably, PEARL is universally applicable to a broad range of absorbers across various spectral windows, encompassing both vibrational and electronic absorptions. Additionally, we employ a femtosecond Time-Resolved Coherent Anti-Stokes Raman Scattering (T-CARS) to scrutinize the temporal characteristics of molecular vibrations. Our findings reveal vibrational dephasing as an emerging indicator capable of discerning subtle alterations in molecular structure and dynamics. This dual approach of extracting temporal features from diverse optical processes opens new vistas for detection and sensing across an array of scientific domains.

Conference Presentation

Citation Download Citation

Delong Zhang "Fluorescence-free super-resolution imaging and nano-environment sensing by assessing temporal evolutions of optical processes", Proc. SPIE PC12853, High-Speed Biomedical Imaging and Spectroscopy IX, PC128530D (13 March 2024); https://doi.org/10.1117/12.3009658

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