Broadband coherent Raman platform for stimulated Raman histology

Benedetta Talone; Francesco Crisafi; Andrea Ragni; Gabriele Di Noia; Mujeeb Rahman; Tiago Azevedo; Junwei Yang; Ivo Petrov; Moe Vali; Renzo Vanna; David Pertzborn; Franziska Hoffmann; Orlando Guntinas-Lichius; Pietro Liò; Andrea C. Ferrari; Giulio Cerullo; Matteo Negro

doi:10.1117/12.3002725

13 March 2024 Broadband coherent Raman platform for stimulated Raman histology

Benedetta Talone, Francesco Crisafi, Andrea Ragni, Gabriele Di Noia, Mujeeb Rahman, Tiago Azevedo, Junwei Yang, Ivo Petrov, Moe Vali, Renzo Vanna, David Pertzborn, Franziska Hoffmann, Orlando Guntinas-Lichius, Pietro Liò, Andrea C. Ferrari, Giulio Cerullo, Matteo Negro

Author Affiliations +

Proceedings Volume PC12855, Advanced Chemical Microscopy for Life Science and Translational Medicine 2024; PC128550W (2024) https://doi.org/10.1117/12.3002725
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Stimulated Raman Histology (SRH) can be used for label-free histological tissue analysis. However the current approaches are limited to two Raman peaks, thus restricting chemically-specific insights. We present a broadband coherent Raman platform (CRP), which overcomes these limitations combining an all-fiber dual output laser and a multichannel lock-in amplifier. This enables the simultaneous detection of 38 channels across the entire CH spectrum (2800-3100 cm-1) in parallel, facilitating chemometric and multimodal tissue analysis. By incorporating AI-driven virtual H&E staining and tissue segmentation for diagnostic purposes, it advances SRH towards clinical-diagnostic.

Conference Presentation

Citation Download Citation

Benedetta Talone, Francesco Crisafi, Andrea Ragni, Gabriele Di Noia, Mujeeb Rahman, Tiago Azevedo, Junwei Yang, Ivo Petrov, Moe Vali, Renzo Vanna, David Pertzborn, Franziska Hoffmann, Orlando Guntinas-Lichius, Pietro Liò, Andrea C. Ferrari, Giulio Cerullo, and Matteo Negro "Broadband coherent Raman platform for stimulated Raman histology", Proc. SPIE PC12855, Advanced Chemical Microscopy for Life Science and Translational Medicine 2024, PC128550W (13 March 2024); https://doi.org/10.1117/12.3002725

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
PRESENTATION

WATCH
PRESENTATION SAVE TO MY LIBRARY

GET CITATION

KEYWORDS

Raman spectroscopy

Raman scattering

Tissues

Coherence imaging

Histopathology

Optical coherence

Spatial resolution

Show All Keywords

Keywords/Phrases

Search In:

Publication Years