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13 March 2024 Nanoscale structural mapping of protein aggregates in live cells modeling Huntington's disease by mid-infrared photothermal microscopy
Zhongyue Guo, Giulio Chiesa, Jiaze Yin, Adam Sanford, Stefan Meier, Ahmad S. Khalil, Ji-Xin Cheng
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Proceedings Volume PC12855, Advanced Chemical Microscopy for Life Science and Translational Medicine 2024; PC128550Z (2024) https://doi.org/10.1117/12.3000552
Event: SPIE BiOS, 2024, San Francisco, California, United States
Abstract
Protein aggregation is associated with many neurodegenerative diseases. We applied mid-infrared photothermal (MIP) to dissect the secondary structure of protein aggregates in living yeast cells modeling Huntington’s disease. We validated that MIP spectroscopy could reveal β-sheet enrichment by significant spectral changes and confirmed the β-sheet enrichment of aggregates in high throughput by wide-field fluorescence-detected MIP. By label-free identification of protein aggregates, we observed a further red shift in spectra for aggregates without GFP tag. Finally, by performing MIP spectroscopy with fine spatial steps, we discovered a partition of secondary structures between β-rich core and ɑ-rich shell of the aggregates.
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Zhongyue Guo, Giulio Chiesa, Jiaze Yin, Adam Sanford, Stefan Meier, Ahmad S. Khalil, and Ji-Xin Cheng "Nanoscale structural mapping of protein aggregates in live cells modeling Huntington's disease by mid-infrared photothermal microscopy", Proc. SPIE PC12855, Advanced Chemical Microscopy for Life Science and Translational Medicine 2024, PC128550Z (13 March 2024); https://doi.org/10.1117/12.3000552
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KEYWORDS
Proteins
Mid-IR
Neurological disorders
Associative arrays
Diseases and disorders
Optical microscopy
Spatial resolution
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