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13 March 2024 Click-free imaging of trehalose trafficking in live mycobacteria via azido photothermal probe (app)
Qing Xia, Harini A Perera, Jiaze Yin, Mingdi Yan, Ji-Xin Cheng
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Proceedings Volume PC12855, Advanced Chemical Microscopy for Life Science and Translational Medicine 2024; PC1285514 (2024) https://doi.org/10.1117/12.3000394
Event: SPIE BiOS, 2024, San Francisco, California, United States
Abstract
Real-time tracking of intracellular carbohydrates remains a grand challenge. While click chemistry enables bio-orthogonal tagging of small molecules with azido groups, subsequent fluorescence visualization is hindered by larger fluorophores, potentially altering intrinsic metabolism. Here, we introduce a click-free, dye-free method for dynamic tracking of trehalose within single mycobacteria via mid-infrared photothermal (MIP) imaging of azido-trehalose. Through spectroscopic MIP imaging, the metabolic pathways of trehalose and its interactions with intracellular proteins is demonstrated without click reaction and photobleaching, offering valuable insights into mycobacterial physiology. Our approach paves the way for further advancements in studying metabolic dynamics within living cells.
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Qing Xia, Harini A Perera, Jiaze Yin, Mingdi Yan, and Ji-Xin Cheng "Click-free imaging of trehalose trafficking in live mycobacteria via azido photothermal probe (app)", Proc. SPIE PC12855, Advanced Chemical Microscopy for Life Science and Translational Medicine 2024, PC1285514 (13 March 2024); https://doi.org/10.1117/12.3000394
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KEYWORDS
Fluorophores
Molecules
Mode conditioning cables
Photobleaching
Plasma
Proteins
Spatial resolution
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