Kilohertz 3-D fluorescent microscopy with light field tomography

Zhaoqiang Wang; Tzung K. Hsiai; Liang Gao

doi:10.1117/12.3001953

13 March 2024 Kilohertz 3-D fluorescent microscopy with light field tomography

Zhaoqiang Wang, Tzung K. Hsiai, Liang Gao

Proceedings Volume PC12857, Computational Optical Imaging and Artificial Intelligence in Biomedical Sciences; PC128571E (2024) https://doi.org/10.1117/12.3001953
Event: SPIE BiOS, 2024, San Francisco, California, United States

Conference Poster

Abstract

Compressed sensing has emerged as a promising technique for high-speed 3-D imaging. Recent work proposed light field tomography (LIFT) to acquire en-face one-dimension (1-D) projections instead of 2-D images and reformulate as a computed tomography problem. The light field with reduced dimension brings high temporal resolution and synthetic refocusing ability in post-processing. We hereby propose a scanning light sheet system with LIFT detection, specifically tailored for achieving kilohertz 3-D fluorescent microscopy. The selective illumination introduces signal sparsity, and thus better reconstruction quality for the compressive detection system. And the high-speed light field imager replaces the active focusing unit in scanning light sheet system and increases the volume rate of 3D detection.

Citation Download Citation

Zhaoqiang Wang, Tzung K. Hsiai, and Liang Gao "Kilohertz 3-D fluorescent microscopy with light field tomography", Proc. SPIE PC12857, Computational Optical Imaging and Artificial Intelligence in Biomedical Sciences, PC128571E (13 March 2024); https://doi.org/10.1117/12.3001953

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