Real-time precision opto-control (RPOC) for manipulating chemical processes in live cells

Matthew G. Clark; Gil Gonzalez; Yiyang Luo; Jesus Aldana-Mendoza; Mark Carlsen; Gregory Eakins; Mingjie Dai; Chi Zhang

doi:10.1117/12.3001121

13 March 2024 Real-time precision opto-control (RPOC) for manipulating chemical processes in live cells

Matthew G. Clark, Gil Gonzalez, Yiyang Luo, Jesus Aldana-Mendoza, Mark Carlsen, Gregory Eakins, Mingjie Dai, Chi Zhang

Proceedings Volume PC12875, Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XXIV; PC1287504 (2024) https://doi.org/10.1117/12.3001121
Event: SPIE LASE, 2024, San Francisco, California, United States

Abstract

Spatiotemporally precise control over complex biological processes in live cells is a long-sought-after goal for researchers. Currently, limited methodologies exist that have the chemical selectivity, spatial precision, or temporal response needed to image and manipulate dynamic biological processes simultaneously. We develop a novel technology, real-time precision opto-control (RPOC), that uses the optical signal generated during laser scanning imaging to activate lasers and control the chemical processes only at the desired pixel locations. We demonstrate the ability of RPOC to precisely manipulate cellular dynamics and as a versatile microsurgery platform for biological applications using a femtosecond laser source.

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Matthew G. Clark, Gil Gonzalez, Yiyang Luo, Jesus Aldana-Mendoza, Mark Carlsen, Gregory Eakins, Mingjie Dai, and Chi Zhang "Real-time precision opto-control (RPOC) for manipulating chemical processes in live cells", Proc. SPIE PC12875, Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XXIV, PC1287504 (13 March 2024); https://doi.org/10.1117/12.3001121

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KEYWORDS

Chemical reactions

Digital filtering

Optical tweezers

Spatial resolution

Molecules

Optical circuits

Optical filters

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