Oil immersed single objective light sheet microscope

Quentin Bécar; Paul Montgomery; Amir Nahas; Vincent Maioli

doi:10.1117/12.3016948

20 June 2024 Oil immersed single objective light sheet microscope

Quentin Bécar, Paul Montgomery, Amir Nahas, Vincent Maioli

Proceedings Volume PC13006, Biomedical Spectroscopy, Microscopy, and Imaging III; PC1300617 (2024) https://doi.org/10.1117/12.3016948
Event: SPIE Photonics Europe, 2024, Strasbourg, France

Abstract

Imaging a large number of samples is necessary to improve statistical robustness in biological assays. Using standard multiwell plates is not possible in usual light-sheet microscopes. One solution is to use an Oblique Plane Microscope (OPM), which is a special fluorescence light sheet microscope, with a single objective near to the sample. To avoid aberrations, OPM generally uses water or silicon immersion to match the refractive index of the sample. In this work we present an oil-immersed OPM and experimentally demonstrate the possibility of using a primary objective with a higher numerical aperture.

Conference Presentation

Citation Download Citation

Quentin Bécar, Paul Montgomery, Amir Nahas, and Vincent Maioli "Oil immersed single objective light sheet microscope", Proc. SPIE PC13006, Biomedical Spectroscopy, Microscopy, and Imaging III, PC1300617 (20 June 2024); https://doi.org/10.1117/12.3016948

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