Optical Clearing of Tissues and Blood

Author(s): Valery V. Tuchin

Published: 2005

PDF ISBN: 9780819481108 | Print ISBN: 9780819460066

DESCRIPTION

This book describes an optical clearing method based on reversible reduction of tissue scattering due to refractive index matching of scatterers and ground matter. This technique, which has been of great interest for research and application in the last decade, is a promising technique for future developments in the fields of tissue imaging, spectroscopy, phototherapy, and laser surgery.
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Front Matter

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CHAPTER 1.

Tissue and Blood Optical Immersion by Exogenous Chemical Agents

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1.1 Tissue Structure and Scattering Properties
1.2 Principles of Optical Immersion and Chemical Agent Diffusion
1.3 Thermodynamics of Water Transport Through a Semipermeable Membrane
1.4 Tissue Swelling and Hydration

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CHAPTER 2.

Optical Clearing of Fibrous Tissues

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2.1 Spectral Properties of Immersed Eye Sclera
2.1.1 Monte Carlo modeling
2.1.2 In vitro measurements
2.2 In vitro Frequency-Domain Measurements of Eye Sclera
2.3 In vivo Measurements of Eye Sclera
2.4 Dura Mater Immersion and Agent Diffusion Rate
2.5 Conclusions

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CHAPTER 3.

Optical Clearing of Skin

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3.1 Introduction
3.2 In vitro Speckle and Spectral Measurements
3.3 In vivo Spectral Reflectance Measurements
3.4 In vivo Frequency-Domain Measurements
3.5 OCT Imaging

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CHAPTER 4.

Optical Clearing of Gastric Tissue

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4.1 Spectral Measurements
4.2 OCT Imaging

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CHAPTER 5.

Tissue Structural Properties Studies at Optical Immersion

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5.1 Polarization Measurements
5.2 Confocal Microscopy
5.3 Fluorescence Measurements
5.3.1 Transmittance of fluorescent radiation
5.3.2 In vivo measurements for intact human skin
5.3.3 Two-photon scanning fluorescence microscopy
5.4 The Second Harmonic Generation

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CHAPTER 6.

Optical Clearing of Blood, Cells, and Microorganisms

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6.1 Blood Flow and Microvessel Network Imaging
6.2 OCT Measurements of Blood Optical Clearing
6.3 Theoretical Analysis of Blood Optical Clearing
6.4 Optical Immersion of Cells and Microorganisms

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CHAPTER 7.

Chemical Agent Delivery

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7.1 Introduction
7.2 Cosmetic Preparations and Skin Permeation
7.3 Diffusion of Macromolecules in Tissues
7.4 Enhanced Diffusion of Molecules Through Tissues
7.4.1 Occlusion
7.4.2 Chemical enhancers
7.4.3 Physical enhancers
7.5 Skin Reservoirs for Topically Applied Clearing Agents

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CHAPTER 8.

Applications

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8.1 Glucose Sensing
8.1.1 Introduction
8.1.2 Tissue and blood scattering spectroscopyv
8.1.3 OCT measurements
8.2 Speckle Technologies
8.2.1 Speckle topography and tomography
8.2.2 Diffusion-wave spectroscopy and functional imaging
8.3 Precision Tissue Photodisruption and Tattoo Removal

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CHAPTER 9.

Other Methods of Tissue Optical Properties Control

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9.1 Tissue Compression and Stretching
9.2 Temperature Effects and Tissue Coagulation
9.3 Tissue Whitening

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Back Matter

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