Fluorescence-enhanced optical tomography of a large tissue phantom using point illumination geometries

Ranadhir Roy; Anuradha Godavarty; Eva Marie Sevick-Muraca

doi:10.1117/1.2338807

1 July 2006 Fluorescence-enhanced optical tomography of a large tissue phantom using point illumination geometries

Ranadhir Roy, Anuradha Godavarty, Eva Marie Sevick-Muraca

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Journal of Biomedical Optics, Vol. 11, Issue 4, 044007 (July 2006). https://doi.org/10.1117/1.2338807

Abstract

We demonstrate fluorescence-enhanced optical imaging of single and multiple fluorescent targets within a large (~1081 cm³) phantom using frequency-domain photon migration measurements of fluorescence collected at individual points in response to illumination of excitation light at individual points on the boundary. The tissue phantom was filled with a 1% lipid solution with and without 0.01 µM Indocyanine Green (ICG) and targets consisted of vials filled with the 1% lipid containing 1–2.5 µM ICG. Measurements were acquired using a modulated intensified CCD imaging system under different experimental conditions. For 3-D image reconstruction, the gradient-based penalty modified barrier function (PMBF) method with simple bounds constrained truncated Newton with trust region method (CONTN) was used. Targets of 0.5, 0.6, and 1.0 cm³ at depths of 1.4–2.8 cm from the phantom surface were tomographically reconstructed. This work demonstrates the practicality of fluorescence-enhanced tomography in clinically relevant volumes.

©(2006) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Ranadhir Roy, Anuradha Godavarty, and Eva Marie Sevick-Muraca "Fluorescence-enhanced optical tomography of a large tissue phantom using point illumination geometries," Journal of Biomedical Optics 11(4), 044007 (1 July 2006). https://doi.org/10.1117/1.2338807

Published: 1 July 2006

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KEYWORDS

Absorption

Reconstruction algorithms

Optical tomography

Luminescence

Spherical lenses

Detection and tracking algorithms

Image restoration

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