Photoacoustic imaging of lacZ gene expression in vivo

Li Li; Roger J. Zemp; Gina F. Lungu; George Stoica D.V.M.; Lihong V. Wang

doi:10.1117/1.2717531

1 March 2007 Photoacoustic imaging of lacZ gene expression in vivo

Li Li, Roger J. Zemp, Gina F. Lungu, George Stoica D.V.M., Lihong V. Wang

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Journal of Biomedical Optics, Vol. 12, Issue 2, 020504 (March 2007). https://doi.org/10.1117/1.2717531

Abstract

In the postgenomic era, imaging techniques are playing an important role in visualizing gene expression in vivo. This work represents the first demonstration of photoacoustic tomography (PAT) for reporter gene imaging. Rats inoculated with 9L/lacZ gliosarcoma tumor cells are imaged with PAT before and after injection of X-gal, a colorimetric assay for the lacZ-encoded enzyme β-galactosidase. Using far-red optical illumination, the genetically tagged tumors in rats are clearly visualized by PAT following the assay. The spatial resolution is quantified to be less than 400 μm, while 500-nM-level sensitivity is demonstrated. With the future development of new absorption-based reporter gene systems, it is anticipated that photoacoustic technology will provide a valuable tool for molecular imaging research.

©(2007) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Li Li, Roger J. Zemp, Gina F. Lungu, George Stoica D.V.M., and Lihong V. Wang "Photoacoustic imaging of lacZ gene expression in vivo," Journal of Biomedical Optics 12(2), 020504 (1 March 2007). https://doi.org/10.1117/1.2717531

Published: 1 March 2007

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