Fiber-laser-based photoacoustic microscopy and melanoma cell detection

Yu Wang; Konstantin I. Maslov; Yu Zhang; Song Hu; Lih-Mei Yang; Younan Xia; Jian Liu; Lihong V. Wang

doi:10.1117/1.3525643

1 January 2011 Fiber-laser-based photoacoustic microscopy and melanoma cell detection

Yu Wang, Konstantin I. Maslov, Yu Zhang, Song Hu, Lih-Mei Yang, Younan Xia, Jian Liu, Lihong V. Wang

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Journal of Biomedical Optics, Vol. 16, Issue 1, 011014 (January 2011). https://doi.org/10.1117/1.3525643

Abstract

For broad applications in biomedical research involving functional dynamics and clinical studies, a photoacoustic microscopy system should be compact, stable, and fast. In this work, we use a fiber laser as the photoacoustic irradiation source to meet these goals. The laser system measures 45×56×13 cm³. The stability of the laser is attributed to the intrinsic optical fiber-based light amplification and output coupling. Its 50-kHz pulse repetition rate enables fast scanning or extensive signal averaging. At the laser wavelength of 1064 nm, the photoacoustic microscope still has enough sensitivity to image small blood vessels while providing high optical absorption contrast between melanin and hemoglobin. Label-free melanoma cells in flowing bovine blood are imaged in vitro, yielding measurements of both cell size and flow speed.

©(2011) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Yu Wang, Konstantin I. Maslov, Yu Zhang, Song Hu, Lih-Mei Yang, Younan Xia, Jian Liu, and Lihong V. Wang "Fiber-laser-based photoacoustic microscopy and melanoma cell detection," Journal of Biomedical Optics 16(1), 011014 (1 January 2011). https://doi.org/10.1117/1.3525643

Published: 1 January 2011

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