Picosecond spectral coherent anti-Stokes Raman scattering imaging with principal component analysis of meibomian glands

Chia-Yu Lin; Eric O. Potma; Jeffrey L. Suhalim; Chyong Ly Nien; James V. Jester; Miloš D. Miljkovic; Max Diem

doi:10.1117/1.3533716

1 February 2011 Picosecond spectral coherent anti-Stokes Raman scattering imaging with principal component analysis of meibomian glands

Chia-Yu Lin, Eric O. Potma, Jeffrey L. Suhalim, Chyong Ly Nien, James V. Jester, Miloš D. Miljkovic, Max Diem

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Journal of Biomedical Optics, Vol. 16, Issue 2, 021104 (February 2011). https://doi.org/10.1117/1.3533716

Abstract

The lipid distribution in the mouse meibomian gland was examined with picosecond spectral anti-Stokes Raman scattering (CARS) imaging. Spectral CARS data sets were generated by imaging specific localized regions of the gland within tissue sections at consecutive Raman shifts in the CH₂ stretching vibrational range. Spectral differences between the location specific CARS spectra obtained in the lipid-rich regions of the acinus and the central duct were observed, which were confirmed with a Raman microspectroscopic analysis, and attributed to meibum lipid modifications within the gland. A principal component analysis of the spectral data set reveals changes in the CARS spectrum when transitioning from the acini to the central duct. These results demonstrate the utility of picosecond spectral CARS imaging combined with multivariate analysis for assessing differences in the distribution and composition of lipids in tissues.

©(2011) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Chia-Yu Lin, Eric O. Potma, Jeffrey L. Suhalim, Chyong Ly Nien, James V. Jester, Miloš D. Miljkovic, and Max Diem "Picosecond spectral coherent anti-Stokes Raman scattering imaging with principal component analysis of meibomian glands," Journal of Biomedical Optics 16(2), 021104 (1 February 2011). https://doi.org/10.1117/1.3533716

Published: 1 February 2011

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