Label-free oxygen-metabolic photoacoustic microscopy in vivo

Junjie Yao; Konstantin I. Maslov; Yu Zhang; Younan Xia; Lihong V. Wang

doi:10.1117/1.3594786

1 July 2011 Label-free oxygen-metabolic photoacoustic microscopy in vivo

Junjie Yao, Konstantin I. Maslov, Yu Zhang, Younan Xia, Lihong V. Wang

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Journal of Biomedical Optics, Vol. 16, Issue 7, 076003 (July 2011). https://doi.org/10.1117/1.3594786

Abstract

Almost all diseases, especially cancer and diabetes, manifest abnormal oxygen metabolism. Accurately measuring the metabolic rate of oxygen (MRO₂) can be helpful for fundamental pathophysiological studies, and even early diagnosis and treatment of disease. Current techniques either lack high resolution or rely on exogenous contrast. Here, we propose label-free metabolic photoacoustic microscopy (mPAM) with small vessel resolution to noninvasively quantify MRO₂in vivo in absolute units. mPAM is the unique modality for simultaneously imaging all five anatomical, chemical, and fluid-dynamic parameters required for such quantification: tissue volume, vessel cross-section, concentration of hemoglobin, oxygen saturation of hemoglobin, and blood flow speed. Hyperthermia, cryotherapy, melanoma, and glioblastoma were longitudinally imaged in vivo. Counterintuitively, increased MRO₂ does not necessarily cause hypoxia or increase oxygen extraction. In fact, early-stage cancer was found to be hyperoxic despite hypermetabolism.

©(2011) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Junjie Yao, Konstantin I. Maslov, Yu Zhang, Younan Xia, and Lihong V. Wang "Label-free oxygen-metabolic photoacoustic microscopy in vivo," Journal of Biomedical Optics 16(7), 076003 (1 July 2011). https://doi.org/10.1117/1.3594786

Published: 1 July 2011

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