Photoacoustic microscopy of tyrosinase reporter gene in vivo

Arie Krumholz; Junjie Yao; Lihong V. Wang; Sarah J. vanVickle-Chavez; Timothy Fleming; William E. Gillanders

doi:10.1117/1.3606568

1 August 2011 Photoacoustic microscopy of tyrosinase reporter gene in vivo

Arie Krumholz, Junjie Yao, Lihong V. Wang, Sarah J. vanVickle-Chavez, Timothy Fleming, William E. Gillanders

Author Affiliations +

Journal of Biomedical Optics, Vol. 16, Issue 8, 080503 (August 2011). https://doi.org/10.1117/1.3606568

Abstract

Photoacoustic tomography is a hybrid modality based on optical absorption excitation and ultrasonic detection. It is sensitive to melanin, one of the primary absorbers in skin. For cells that do not naturally contain melanin, melanin production can be induced by introducing the gene for tyrosinase, the primary enzyme responsible for expression of melanin in melanogenic cells. Optical resolution photoacoustic microscopy was used in the ex vivo study reported here, where the signal from transfected cells increased by more than 10 times over wild-type cells. A subsequent in vivo experiment was conducted to demonstrate the capability of photoacoustic microscopy to spectrally differentiate between tyrosinase-catalyzed melanin and various other absorbers in tissue.

©(2011) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Arie Krumholz, Junjie Yao, Lihong V. Wang, Sarah J. vanVickle-Chavez, Timothy Fleming, and William E. Gillanders "Photoacoustic microscopy of tyrosinase reporter gene in vivo," Journal of Biomedical Optics 16(8), 080503 (1 August 2011). https://doi.org/10.1117/1.3606568

Published: 1 August 2011

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KEYWORDS

Blood

Absorption

Acquisition tracking and pointing

In vivo imaging

Photoacoustic microscopy

Ultrasonics

Signal to noise ratio

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