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3 August 2012 Low-level laser therapy on MCF-7 cells: a micro-Fourier transform infrared spectroscopy study
Taciana D. Magrini, Nathalia Villa dos Santos, Marcella P. Milazzotto, Giselle Cerchiaro, Herculano S. da Silva Martinho
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Journal of Biomedical Optics, Vol. 17, Issue 10, 101516 (August 2012). https://doi.org/10.1117/1.JBO.17.10.101516
Abstract
Low-level laser therapy (LLLT) is an emerging therapeutic approach for several clinical conditions. The clinical effects induced by LLLT presumably scale from photobiostimulation/photobioinhibition at the cellular level to the molecular level. The detailed mechanism underlying this effect remains unknown. This study quantifies some relevant aspects of LLLT related to molecular and cellular variations. Malignant breast cells (MCF-7) were exposed to spatially filtered light from a He-Ne laser (633 nm) with fluences of 5, 28.8, and 1000  mJ/cm2. The cell viability was evaluated by optical microscopy using the Trypan Blue viability test. The micro-Fourier transform infrared technique was employed to obtain the vibrational spectra of each experimental group (control and irradiated) and identify the relevant biochemical alterations that occurred due to the process. It was observed that the red light influenced the RNA, phosphate, and serine/threonine/tyrosine bands. We found that light can influence cell metabolism depending on the laser fluence. For 5  mJ/cm2, MCF-7 cells suffer bioinhibition with decreased metabolic rates. In contrast, for the 1  J/cm2 laser fluence, cells present biostimulation accompanied by a metabolic rate elevation. Surprisingly, at the intermediate fluence, 28.8  mJ/cm2, the metabolic rate is increased despite the absence of proliferative results. The data were interpreted within the retrograde signaling pathway mechanism activated with light irradiation.
© 2012 Society of Photo-Optical Instrumentation Engineers (SPIE) 0091-3286/2012/$25.00 © 2012 SPIE
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Taciana D. Magrini, Nathalia Villa dos Santos, Marcella P. Milazzotto, Giselle Cerchiaro, and Herculano S. da Silva Martinho "Low-level laser therapy on MCF-7 cells: a micro-Fourier transform infrared spectroscopy study," Journal of Biomedical Optics 17(10), 101516 (3 August 2012). https://doi.org/10.1117/1.JBO.17.10.101516
Published: 3 August 2012
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KEYWORDS
Laser therapeutics
Proteins
Mode conditioning cables
FT-IR spectroscopy
Infrared radiation
Infrared spectroscopy
Spectroscopy
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