Investigation of lipid homeostasis in living Drosophila by coherent anti-Stokes Raman scattering microscopy

Cheng-Hao Chien; Wei-Wen Chen; June-Tai Wu; Ta-Chau Chang

doi:10.1117/1.JBO.17.12.126001

3 December 2012 Investigation of lipid homeostasis in living Drosophila by coherent anti-Stokes Raman scattering microscopy

Cheng-Hao Chien, Wei-Wen Chen, June-Tai Wu, Ta-Chau Chang

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Journal of Biomedical Optics, Vol. 17, Issue 12, 126001 (December 2012). https://doi.org/10.1117/1.JBO.17.12.126001

Abstract

To improve our understanding of lipid metabolism, Drosophila is used as a model animal, and its lipid homeostasis is monitored by coherent anti-Stokes Raman scattering microscopy. We are able to achieve in vivo imaging of larval fat body (analogous to adipose tissue in mammals) and oenocytes (analogous to hepatocytes) in Drosophila larvae at subcellular level without any labeling. By overexpressing two lipid regulatory proteins-Brummer lipase (Bmm) and lipid storage droplet-2 (Lsd-2)-we found different phenotypes and responses under fed and starved conditions. Comparing with the control larva, we observed more lipid droplet accumulation by ~twofold in oenocytes of fat-body-Bmm-overexpressing (FB-Bmm-overexpressing) mutant under fed condition, and less lipid by ~fourfold in oenocytes of fat-body-Lsd-2-overexpressing (FB-Lsd-2-overexpressing) mutant under starved condition. Moreover, together with reduced size of lipid droplets, the lipid content in the fat body of FB-Bmm-overexpressing mutant decreases much faster than that of the control and FB-Lsd-2-overexpressing mutant during starvation. From long-term starvation assay, we found FB-Bmm-overexpressing mutant has a shorter lifespan, which can be attributed to faster consumption of lipid in its fat body. Our results demonstrate in vivo observations of direct influences of Bmm and Lsd-2 on lipid homeostasis in Drosophila larvae.

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Cheng-Hao Chien, Wei-Wen Chen, June-Tai Wu, and Ta-Chau Chang "Investigation of lipid homeostasis in living Drosophila by coherent anti-Stokes Raman scattering microscopy," Journal of Biomedical Optics 17(12), 126001 (3 December 2012). https://doi.org/10.1117/1.JBO.17.12.126001

Published: 3 December 2012

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