Detection of brain tumors using fluorescence diffuse optical tomography and nanoparticles as contrast agents

Pierre-Yves Fortin; Coralie Genevois; Anne Koenig; Emilie Heinrich; Isabelle Texier-Nogues; Franck Couillaud

doi:10.1117/1.JBO.17.12.126004

3 December 2012 Detection of brain tumors using fluorescence diffuse optical tomography and nanoparticles as contrast agents

Pierre-Yves Fortin, Coralie Genevois, Anne Koenig, Emilie Heinrich, Isabelle Texier-Nogues, Franck Couillaud

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Journal of Biomedical Optics, Vol. 17, Issue 12, 126004 (December 2012). https://doi.org/10.1117/1.JBO.17.12.126004

Abstract

Near-infrared fluorescence-enhanced diffuse optical tomography (fDOT) is used to localize tumors in mice using fluorescent nanoparticles as a blood pool contrast agent. The infrared dye DiR is loaded in the lipid core of nontargeted nanoparticles (DiR-lipidots) and injected systemically via the tail vein in mice bearing U87 tumors. Distribution and time-course of DiR-lipidots are followed using in vivo fluorescence reflectance imaging and reveal enhanced fluorescent signal within the subcutaneous tumors up to seven days due to the enhanced permeability and retention effect. Tumor growth into the brain is followed using bioluminescent imaging, and tumor localization is further determined by magnetic resonance imaging. The fDOT provides three-dimensional fluorescent maps that allow for consistent localization for both subcutaneous and brain tumors.

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Pierre-Yves Fortin, Coralie Genevois, Anne Koenig, Emilie Heinrich, Isabelle Texier-Nogues, and Franck Couillaud "Detection of brain tumors using fluorescence diffuse optical tomography and nanoparticles as contrast agents," Journal of Biomedical Optics 17(12), 126004 (3 December 2012). https://doi.org/10.1117/1.JBO.17.12.126004

Published: 3 December 2012

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