Functional photoacoustic microscopy of diabetic vasculature

Arie Krumholz; Lidai Wang; Junjie Yao; Lihong V. Wang

doi:10.1117/1.JBO.17.6.060502

21 May 2012 Functional photoacoustic microscopy of diabetic vasculature

Arie Krumholz, Lidai Wang, Junjie Yao, Lihong V. Wang

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Journal of Biomedical Optics, Vol. 17, Issue 6, 060502 (May 2012). https://doi.org/10.1117/1.JBO.17.6.060502

Abstract

We used functional photoacoustic microscopy to image diabetes-induced damage to the microvasculature. To produce an animal model for Type 1 diabetes, we used streptozotocin (STZ), which is particularly toxic to the insulin-producing beta cells of the pancreas in mammals. A set number of ND4 Swiss Webster mice received intraperitoneal injections of STZ for five consecutive days at 50 mg/kg. Most mice developed a significant rise in blood glucose level ( ∼ 400 mg/dL) within three weeks of the first injection. Changes in vasculature and hemodynamics were monitored for six weeks. The mouse ear was imaged with an optical-resolution photoacoustic microscope at a main blood vessel branch from the root of the ear. There are noticeable and measurable changes associated with the disease, including decreased vessel diameter and possible occlusion due to vessel damage and polyurea. We also observed an increase in the blood flow speed in the vein and a decrease in the artery, which could be due to compensation for the dehydration and vessel diameter changes. Functional and metabolic parameters such as hemoglobin oxygen saturation, oxygen extraction fraction, and oxygen consumption rate were also measured, but showed no significant change.

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Arie Krumholz, Lidai Wang, Junjie Yao, and Lihong V. Wang "Functional photoacoustic microscopy of diabetic vasculature," Journal of Biomedical Optics 17(6), 060502 (21 May 2012). https://doi.org/10.1117/1.JBO.17.6.060502

Published: 21 May 2012

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