Discriminating model for diagnosis of basal cell carcinoma and melanoma in vitro based on the Raman spectra of selected biochemicals

Landulfo Silveira Jr.; Fabrício L. Silveira; Renato A. Zângaro M.D.; Marcos T. Pacheco; Benito Bodanese

doi:10.1117/1.JBO.17.7.077003

6 July 2012 Discriminating model for diagnosis of basal cell carcinoma and melanoma in vitro based on the Raman spectra of selected biochemicals

Landulfo Silveira Jr., Fabrício L. Silveira, Renato A. Zângaro M.D., Marcos T. Pacheco, Benito Bodanese

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Journal of Biomedical Optics, Vol. 17, Issue 7, 077003 (July 2012). https://doi.org/10.1117/1.JBO.17.7.077003

Abstract

Raman spectroscopy has been employed to identify differences in the biochemical constitution of malignant [basal cell carcinoma (BCC) and melanoma (MEL)] cells compared to normal skin tissues, with the goal of skin cancer diagnosis. We collected Raman spectra from compounds such as proteins, lipids, and nucleic acids, which are expected to be represented in human skin spectra, and developed a linear least-squares fitting model to estimate the contributions of these compounds to the tissue spectra. We used a set of 145 spectra from biopsy fragments of normal (30 spectra), BCC (96 spectra), and MEL (19 spectra) skin tissues, collected using a near-infrared Raman spectrometer (830 nm, 50 to 200 mW, and 20 s exposure time) coupled to a Raman probe. We applied the best-fitting model to the spectra of biochemicals and tissues, hypothesizing that the relative spectral contribution of each compound to the tissue Raman spectrum changes according to the disease. We verified that actin, collagen, elastin, and triolein were the most important biochemicals representing the spectral features of skin tissues. A classification model applied to the relative contribution of collagen III, elastin, and melanin using Euclidean distance as a discriminator could differentiate normal from BCC and MEL.

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Landulfo Silveira Jr., Fabrício L. Silveira, Renato A. Zângaro M.D., Marcos T. Pacheco, and Benito Bodanese "Discriminating model for diagnosis of basal cell carcinoma and melanoma in vitro based on the Raman spectra of selected biochemicals," Journal of Biomedical Optics 17(7), 077003 (6 July 2012). https://doi.org/10.1117/1.JBO.17.7.077003

Published: 6 July 2012

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