Wide-field fast-scanning photoacoustic microscopy based on a water-immersible MEMS scanning mirror

Junjie Yao; Lidai Wang; Joon-Mo Yang; Liang S. Gao; Konstantin I. Maslov; Lihong V. Wang; Chih-Hsien Huang; Jun Zou

doi:10.1117/1.JBO.17.8.080505

14 August 2012 Wide-field fast-scanning photoacoustic microscopy based on a water-immersible MEMS scanning mirror

Junjie Yao, Lidai Wang, Joon-Mo Yang, Liang S. Gao, Konstantin I. Maslov, Lihong V. Wang, Chih-Hsien Huang, Jun Zou

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Journal of Biomedical Optics, Vol. 17, Issue 8, 080505 (August 2012). https://doi.org/10.1117/1.JBO.17.8.080505

Abstract

By offering images with high spatial resolution and unique optical absorption contrast, optical-resolution photoacoustic microscopy (OR-PAM) has gained increasing attention in biomedical research. Recent developments in OR-PAM have improved its imaging speed, but have to sacrifice either the detection sensitivity or field of view or both. We have developed a wide-field fast-scanning OR-PAM by using a water-immersible microelectromechanical systems (MEMS) scanning mirror (MEMS-OR-PAM). In MEMS-OR-PAM, the optical and acoustic beams are confocally configured and simultaneously steered, which ensures the uniform detection sensitivity. A B-scan imaging speed as high as 400 Hz can be achieved over a 3 mm scanning range. Using the system, we imaged the flow dynamics of both red blood cells and carbon particles in a mouse ear in vivo. Presented results show that MEMS-OR-PAM could be a powerful tool for studying highly dynamic and time-sensitive biological phenomena.

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Junjie Yao, Lidai Wang, Joon-Mo Yang, Liang S. Gao, Konstantin I. Maslov, Lihong V. Wang, Chih-Hsien Huang, and Jun Zou "Wide-field fast-scanning photoacoustic microscopy based on a water-immersible MEMS scanning mirror," Journal of Biomedical Optics 17(8), 080505 (14 August 2012). https://doi.org/10.1117/1.JBO.17.8.080505

Published: 14 August 2012

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