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2 October 2013 Photoacoustic recovery after photothermal bleaching in living cells
Chiye Li, Chi Zhang, Liang Gao, Alejandro Garcia-Uribe, Lihong V. Wang
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Journal of Biomedical Optics, Vol. 18, Issue 10, 106004 (October 2013). https://doi.org/10.1117/1.JBO.18.10.106004
Abstract
We present an innovative method, photoacoustic recovery after photothermal bleaching (PRAP), for studying particle dynamics at micron scale via photoacoustic imaging. As an intuitive way to visualize and quantify dynamic processes, PRAP is demonstrated first in a simple phantom study and then in a more complex measurement involving live cells. Compared with the conventional fluorescence-based approach, PRAP provides high signal-to-noise ratio (SNR) imaging with minimal bleaching-induced artifacts during the recovery stage, ideal for monitoring the diffusive and kinetic processes inside a cell.
© 2013 Society of Photo-Optical Instrumentation Engineers (SPIE) 0091-3286/2013/$25.00 © 2013 SPIE
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Chiye Li, Chi Zhang, Liang Gao, Alejandro Garcia-Uribe, and Lihong V. Wang "Photoacoustic recovery after photothermal bleaching in living cells," Journal of Biomedical Optics 18(10), 106004 (2 October 2013). https://doi.org/10.1117/1.JBO.18.10.106004
Published: 2 October 2013
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Cited by 8 scholarly publications.
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KEYWORDS
Diffusion
Particles
Photoacoustic spectroscopy
Luminescence
Pulsed laser operation
Microscopes
Signal to noise ratio
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