In vivo dynamic process imaging using real-time optical-resolution photoacoustic microscopy

Wei Shi; Peng Shao; Parsin Hajireza; Alexander Forbrich; Roger J. Zemp

doi:10.1117/1.JBO.18.2.026001

1 February 2013 In vivo dynamic process imaging using real-time optical-resolution photoacoustic microscopy

Wei Shi, Peng Shao, Parsin Hajireza, Alexander Forbrich, Roger J. Zemp

Author Affiliations +

Journal of Biomedical Optics, Vol. 18, Issue 2, 026001 (February 2013). https://doi.org/10.1117/1.JBO.18.2.026001

Abstract

The authors demonstrate in vivo dynamic process imaging using a label-free real-time optical-resolution photoacoustic microscope (OR-PAM). This reflection-mode system takes advantage of a 532-nm fiber laser source with a high pulse repetition rate of up to 600 kHz combined with a fast-scanning mirror system. Microvasculature in SCID mouse ears is imaged at near real-time (0.5 fps) for a 1×1 mm ² field of view (FOV) with micron-scale lateral resolution. We also demonstrate imaging of cardiac-induced microhemodynamics in murine microvasculature at real-time frame-rates (30 fps) over a 250×250 μm ² FOV using real-time C-scan OR-PAM with ability to provide sustained imaging with near real-time feedback for focusing and positioning.

CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Wei Shi, Peng Shao, Parsin Hajireza, Alexander Forbrich, and Roger J. Zemp "In vivo dynamic process imaging using real-time optical-resolution photoacoustic microscopy," Journal of Biomedical Optics 18(2), 026001 (1 February 2013). https://doi.org/10.1117/1.JBO.18.2.026001

Published: 1 February 2013

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Cited by 24 scholarly publications.

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KEYWORDS

Imaging systems

In vivo imaging

Data acquisition

Fiber lasers

Image processing

Real time imaging

Photoacoustic spectroscopy

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