Single-cell photoacoustic thermometry

Liang Gao; Lidai Wang; Chiye Li; Yan Liu; Haixin Ke; Chi Zhang; Lihong V. Wang

doi:10.1117/1.JBO.18.2.026003

1 February 2013 Single-cell photoacoustic thermometry

Liang Gao, Lidai Wang, Chiye Li, Yan Liu, Haixin Ke, Chi Zhang, Lihong V. Wang

Author Affiliations +

Journal of Biomedical Optics, Vol. 18, Issue 2, 026003 (February 2013). https://doi.org/10.1117/1.JBO.18.2.026003

Abstract

A novel photoacoustic thermometric method is presented for simultaneously imaging cells and sensing their temperature. With three-seconds-per-frame imaging speed, a temperature resolution of 0.2°C was achieved in a photo-thermal cell heating experiment. Compared to other approaches, the photoacoustic thermometric method has the advantage of not requiring custom-developed temperature-sensitive biosensors. This feature should facilitate the conversion of single-cell thermometry into a routine lab tool and make it accessible to a much broader biological research community.

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Liang Gao, Lidai Wang, Chiye Li, Yan Liu, Haixin Ke, Chi Zhang, and Lihong V. Wang "Single-cell photoacoustic thermometry," Journal of Biomedical Optics 18(2), 026003 (1 February 2013). https://doi.org/10.1117/1.JBO.18.2.026003

Published: 1 February 2013

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