Using optical profilometry to characterize cell membrane roughness influenced by amyloid-beta 42 aggregates and electric fields

Huei-Jyuan Pan; Ruei-Lin Wang; Jian-Long Xiao; Yu-Jen Chang; Ji-Yen Cheng; Yu-Jen Chang; Chau-Hwang Lee

doi:10.1117/1.JBO.19.1.011009

26 July 2013 Using optical profilometry to characterize cell membrane roughness influenced by amyloid-beta 42 aggregates and electric fields

Huei-Jyuan Pan, Ruei-Lin Wang, Jian-Long Xiao, Yu-Jen Chang, Ji-Yen Cheng, Yu-Jen Chang, Chau-Hwang Lee

Author Affiliations +

Journal of Biomedical Optics, Vol. 19, Issue 1, 011009 (July 2013). https://doi.org/10.1117/1.JBO.19.1.011009

Abstract

The membrane roughness of Neuro-2a neroblastoma cells is measured by using noninterferometric wide-field optical profilometry. The cells are treated with the fibril and oligomer conformers of amyloid-beta (Aβ ) 42, which is a peptide of 42 amino acids related to the development of Alzheimer’s disease. We find that both the Aβ42 fibrils and Aβ42 oligomers reduced the cell membrane roughness, but the effect of Aβ42 oligomers was faster and stronger than that of the fibrils. We also apply direct-current electric field (dcEF) stimulations on the cells. A dcEF of 300 mV/mm can increase the membrane roughness under the treatment of Aβ42 . These results suggest that Aβ42 can decrease the membrane compliance of live neuroblastoma cells, and dcEFs may counteract this effect.

CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

Citation Download Citation

Huei-Jyuan Pan, Ruei-Lin Wang, Jian-Long Xiao, Yu-Jen Chang, Ji-Yen Cheng, Yu-Jen Chang, and Chau-Hwang Lee "Using optical profilometry to characterize cell membrane roughness influenced by amyloid-beta 42 aggregates and electric fields," Journal of Biomedical Optics 19(1), 011009 (26 July 2013). https://doi.org/10.1117/1.JBO.19.1.011009

Published: 26 July 2013

ACCESS THE FULL ARTICLE

JOURNAL ARTICLE
5 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY