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16 June 2014 Raman and coherent anti-Stokes Raman scattering microscopy studies of changes in lipid content and composition in hormone-treated breast and prostate cancer cells
Mariana C. Potcoava, Gregory L. Futia, Jessica Aughenbaugh, Isabel R. Schlaepfer, Emily A. Gibson
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Funded by: American Cancer Society Institutional Research, Defense Advanced Research Projects Agency, NIH National Cancer Institute, NIH/NCATS Colorado CTSI
Journal of Biomedical Optics, Vol. 19, Issue 11, 111605 (June 2014). https://doi.org/10.1117/1.JBO.19.11.111605
Abstract
Increasing interest in the role of lipids in cancer cell proliferation and resistance to drug therapies has motivated the need to develop better tools for cellular lipid analysis. Quantification of lipids in cells is typically done by destructive chromatography protocols that do not provide spatial information on lipid distribution and prevent dynamic live cell studies. Methods that allow the analysis of lipid content in live cells are therefore of great importance. Using micro-Raman spectroscopy and coherent anti-Stokes Raman scattering (CARS) microscopy, we generated a lipid profile for breast (T47D, MDA-MB-231) and prostate (LNCaP, PC3) cancer cells upon exposure to medroxyprogesterone acetate (MPA) and synthetic androgen R1881. Combining Raman spectra with CARS imaging, we can study the process of hormone-mediated lipogenesis. Our results show that hormone-treated cancer cells T47D and LNCaP have an increased number and size of intracellular lipid droplets and higher degree of saturation than untreated cells. MDA-MB-231 and PC3 cancer cells showed no significant changes upon treatment. Principal component analysis with linear discriminant analysis of the Raman spectra was able to differentiate between cancer cells that were treated with MPA, R1881, and untreated.
CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
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Mariana C. Potcoava, Gregory L. Futia, Jessica Aughenbaugh, Isabel R. Schlaepfer, and Emily A. Gibson "Raman and coherent anti-Stokes Raman scattering microscopy studies of changes in lipid content and composition in hormone-treated breast and prostate cancer cells," Journal of Biomedical Optics 19(11), 111605 (16 June 2014). https://doi.org/10.1117/1.JBO.19.11.111605
Published: 16 June 2014
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KEYWORDS
Raman spectroscopy
Cancer
Microscopy
CARS tomography
Prostate cancer
Breast
Oncology
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