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19 November 2014 Photoacoustic lymphatic imaging with high spatial-temporal resolution
Catherine Martel, Junjie Yao, Jun Zou, Chih-Hsien Huang, Gwendalyn J. Randolph, Lihong V. Wang
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Journal of Biomedical Optics, Vol. 19, Issue 11, 116009 (November 2014). https://doi.org/10.1117/1.JBO.19.11.116009
Abstract
Despite its critical function in coordinating the egress of inflammatory and immune cells out of tissues and maintaining fluid balance, the causative role of lymphatic network dysfunction in pathological settings is still understudied. Engineered-animal models and better noninvasive high spatial-temporal resolution imaging techniques in both preclinical and clinical studies will help to improve our understanding of different lymphatic-related pathologic disorders. Our aim was to take advantage of our newly optimized noninvasive wide-field fast-scanning photoacoustic (PA) microcopy system to coordinately image the lymphatic vasculature and its flow dynamics, while maintaining high resolution and detection sensitivity. Here, by combining the optical-resolution PA microscopy with a fast-scanning water-immersible microelectromechanical system scanning mirror, we have imaged the lymph dynamics over a large field-of-view, with high spatial resolution and advanced detection sensitivity. Depending on the application, lymphatic vessels (LV) were spectrally or temporally differentiated from blood vessels. Validation experiments were performed on phantoms and in vivo to identify the LV. Lymphatic flow dynamics in nonpathological and pathological conditions were also visualized. These results indicate that our newly developed PA microscopy is a promising tool for lymphatic-related biological research.
© 2014 Society of Photo-Optical Instrumentation Engineers (SPIE) 1083-3668/2014/$25.00 © 2014 SPIE
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Catherine Martel, Junjie Yao, Jun Zou, Chih-Hsien Huang, Gwendalyn J. Randolph, and Lihong V. Wang "Photoacoustic lymphatic imaging with high spatial-temporal resolution," Journal of Biomedical Optics 19(11), 116009 (19 November 2014). https://doi.org/10.1117/1.JBO.19.11.116009
Published: 19 November 2014
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KEYWORDS
Lymphatic system
Blood vessels
Image resolution
Photoacoustic spectroscopy
Photoacoustic imaging
Ear
Tissues
CHORUS Article. This article was made freely available starting 19 November 2015
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