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3 March 2014 Integrated photoacoustic, confocal, and two-photon microscope
Bin Rao, Florentina Soto, Daniel Kerschensteiner, Lihong V. Wang
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Journal of Biomedical Optics, Vol. 19, Issue 3, 036002 (March 2014). https://doi.org/10.1117/1.JBO.19.3.036002
Abstract
The invention of green fluorescent protein and other molecular fluorescent probes has promoted applications of confocal and two-photon fluorescence microscopy in biology and medicine. However, exogenous fluorescence contrast agents may affect cellular structure and function, and fluorescence microscopy cannot image nonfluorescent chromophores. We overcome this limitation by integrating optical-resolution photoacoustic microscopy into a modern Olympus IX81 confocal, two-photon, fluorescence microscope setup to provide complementary, label-free, optical absorption contrast. Automatically coregistered images can be generated from the same sample. Imaging applications in ophthalmology, developmental biology, and plant science are demonstrated. For the first time, in a familiar microscopic fluorescence imaging setting, this trimodality microscope provides a platform for future biological and medical discoveries.
© 2014 Society of Photo-Optical Instrumentation Engineers (SPIE) 0091-3286/2014/$25.00 © 2014 SPIE
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Bin Rao, Florentina Soto, Daniel Kerschensteiner, and Lihong V. Wang "Integrated photoacoustic, confocal, and two-photon microscope," Journal of Biomedical Optics 19(3), 036002 (3 March 2014). https://doi.org/10.1117/1.JBO.19.3.036002
Published: 3 March 2014
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Cited by 37 scholarly publications.
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KEYWORDS
Confocal microscopy
Luminescence
Microscopes
Mirrors
Absorption
Microscopy
Photoacoustic spectroscopy
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